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  • Formulation and Evaluation of Anti- Bacterial Moringa Soap

  • 1Research Scholar, Department of Pharmaceutics, Delight College of Pharmacy, Koregaon Bhima, Pune, Maharashtra. India-412216. 
    2Assistant Professor Department of Pharmaceutics, Delight College of Pharmacy, Koregaon Bhima, Pune. Maharashtra, India-412216. 
    3Assistant Professor, Department of Quality Assurance Techniques, Delight College of Pharmacy, Koregaon Bhima, Pune, Maharashtra, India-412216. 
     

Abstract

This study aims to formulate and evaluate moringa soap, utilizing the natural benefits of moringa oil, which is rich in antioxidants, vitamins, and essential fatty acids. The primary goal is to create a skin-care product that nourishes, moisturizes, and improves skin health. Moringa soap is prepared through the cold process method, incorporating moringa oil along with other natural ingredients to enhance its cosmetic and therapeutic properties. The rise of multidrug-resistant bacteria causing infections in humans is steadily increasing, while the development of new medications to counter this issue remains inadequate. Research has shown that Moringa oleifera Lamk possesses multiple medicinal properties, including antibacterial, antifungal, antispasmodic, anti-inflammatory, anticancer, and diuretic effects. As a result, herbal remedies could offer a potential approach to managing infections caused by multidrug-resistant bacteria. Staphylococcus aureus bacteria are commonly found as part of the normal skin flora, but when their numbers exceed the normal limit, they can lead to infection. To help prevent this, maintaining skin cleanliness by using soap with antibacterial agents, like flavonoids from Moringa leaves, is recommended. This study focuses on analyzing the flavonoid content, pH, Foaming Index and Foaming Height in Moringa leaves and incorporating it into transparent solid soap formulations. The soap was prepared with varying concentrations of Moringa leaf ethanol extract: 2.5%, 3.5%, and 4.5%.

Keywords

Moringa oleifera leaf, Antibacterial activity, Moringa soap, skin protection

Introduction

Microorganisms are present all around us and can lead to infections, such as those affecting the skin. These skin infections can be triggered by various types of microorganisms, including bacteria. Although bacteria naturally exist on the skin, an overgrowth of these bacteria can result in an infection. Staphylococcus aureus is one such bacterium that can cause skin infections in humans. This bacterium can be found on various objects like keyboards, wristbands, escalator handrails, and door handles. To prevent bacterial infections, it's important to maintain good personal hygiene, one way of which is by using cleansing products. Soap is a commonly used product for this purpose. Bath soap, which can be in solid, liquid, or foamy form, is a cosmetic product used for cleaning the skin. The effectiveness of bath soap in eliminating germs can be enhanced by incorporating antibacterial ingredients into its formulation. Natural antibacterial components for bath soap formulations can be sourced from the Moringa plant. Moringa leaves are rich in flavonoid compounds. one of which is quercetin. Quercetin has strong antibacterial properties. Considering the link between the flavonoids in Moringa leaves and their antibacterial potential, this study aims to analyze the flavonoid content in Moringa leaf extract and assess its incorporation into antibacterial transparent soap formulations.1 Moringa oleifera, commonly known as the drumstick tree, miracle tree, or horseradish tree, is a highly valued plant native to the Indian subcontinent and widely cultivated across tropical and subtropical regions of the world. It belongs to the family Moringaceae and is renowned for its remarkable nutritional and medicinal properties. All parts of the plant including leaves, pods, seeds, flowers, and roots are utilized in various applications ranging from food and traditional medicine to agriculture and water purification.2 The leaves of Moringa oleifera are especially notable for their high content of essential nutrients such as vitamins A, C, and E, calcium, iron, and protein. Due to its rich phytochemical composition, Moringa oleifera has been extensively studied for its antioxidant, anti-inflammatory, antimicrobial, and antidiabetic activities. As global interest in functional foods and natural remedies continues to rise, Moringa oleifera is gaining attention as a sustainable and multipurpose resource for combating malnutrition and promoting health.3 Moringa, scientifically known as Moringa oleifera, is a fast-growing, drought-resistant tree native to the Indian subcontinent. It is commonly referred to as the “drumstick tree,” “miracle tree,” or “horseradish tree.” Moringa is highly valued for its nutritional, medicinal, and economic benefits.4 Every part of the plant leaves, pods, seeds, flowers, and even roots is edible and rich in nutrients. The leaves are particularly noted for their high content of vitamins (A, C, and E), minerals (especially calcium and iron), and proteins. Due to its health-promoting properties, moringa is widely used in traditional medicine and is gaining popularity globally as a superfood.5

Moringa

What is Moringa?

Scientific name: Moringa oleifera

Moringa is a fast-growing, drought-resistant tree native to the Indian subcontinent. It is widely grown in tropical and subtropical areas for its nutritious leaves, pods, seeds, and flowers.6

Nutritional Value

Moringa leaves are incredibly nutritious. Here’s what they offer per 100g (fresh leaves):

  • Protein: 9g (very high for a plant).
  • Vitamin C: 200mg (more than oranges).
  • Vitamin A: 6,780 IU (more than carrots).
  • Calcium: 185mg (more than milk).
  • Iron: 4mg (more than spinach).
  • Potassium: 337mg.7

Health Benefits

  1. Rich in Antioxidants Contains quercetin, chlorogenic acid, and beta-carotene, which help fight oxidative stress.
  2. Anti-Inflammatory Properties Useful for reducing inflammation in chronic diseases like arthritis.
  3. Blood Sugar Control Some studies show moringa can help lower blood sugar levels.
  4. Cholesterol-Lowering Effects May reduce bad cholesterol (LDL) and improve heart health.
  5. Brain Health Antioxidants and neuroprotective compounds may improve memory and mental clarity.
  6. Antibacterial and Antifungal Effective against certain bacteria and fungi, supporting immune function.8

Fig 1: Moringa Powder

How to Use Moringa

  • Fresh leaves: Cook like spinach in soups, stews, or stir-fries.
  • Dried powder: Add to smoothies, tea, or sprinkle on food.
  • Pods (drumsticks): Common in Indian curries.
  • Seeds: Eaten roasted, or used for water purification.
  • Oil (Ben oil): Used in cosmetics and cooking.9

 Possible Side Effects

  • High doses may cause nausea or diarrhoea.
  • Pregnant women should avoid moringa root and bark (can cause uterine contractions).
  • Always consult with a healthcare provider before taking it as a supplement.10

Global Uses

  • India: Used in Ayurvedic medicine for over 5,000 years.
  • Africa: Grown in dry regions for food security and malnutrition.
  • Philippines and Southeast Asia: Common in soups and health tonics.11Bottom of Form

CAUSES AND RISK FACTORS

  1. Overconsumption
    1. Taking too much moringa powder or extract can lead to digestive problems like nausea, vomiting, and diarrhoea.
    2. Excess intake of iron and calcium from concentrated powder may stress the kidneys in vulnerable people.
  2. Consuming Toxic Parts of the Plant
    1. Roots and bark contain Spiro chin, a potentially toxic alkaloid that may cause nerve paralysis or miscarriage.
    2. Moringa root extract has been linked to harmful effects in animal studies.
  3. Contaminated Products
    1. Poorly processed or stored moringa supplements may be contaminated with bacteria, heavy metals, or pesticides.
    2. Risk is higher with unregulated or imported supplements.
  4. Drug Interactions
    1. Moringa may interact with:
      1. Blood pressure medications (can cause too-low blood pressure).
      2. Diabetes medications (can dangerously lower blood sugar).
      3. Thyroid medications.12

Risk Factors for Experiencing Side Effects

You are more at risk of adverse effects if you:

Risk Factor

Why it Matters

Pregnant or Breastfeeding

Moringa root and bark may cause uterine contractions or toxicity to the baby.

Taking Medications

Moringa can alter how drugs for diabetes, hypertension, and thyroid are metabolized.

Pre-existing Liver or Kidney Disease

High concentrations of minerals and bioactive compounds may burden these organs.

Using Concentrated Extracts

These are much more potent than food forms and can cause stronger side effects.

Children under 2 years

Their developing systems are more sensitive to plant compounds and dosing is unclear.13

Safety Tips

  • Use leaves and pods, not root or bark.
  • Start with small amounts (1 tsp of powder daily).
  • Choose reputable brands with quality testing.
  • Talk to your doctor or pharmacist if you're on medication.
  • Avoid during pregnancy unless advised by a health professional.14

Plant Profile

Moringa leaves

Moringa leaves are the foliage of the Moringa oleifera tree, often referred to as the "drumstick tree" or "miracle tree." These leaves are highly nutritious and are widely used in traditional medicine and cuisine, especially in Asia and Africa.15 

Reference

  1. Ahmad Royani (2023), formulation and evaluation of anti- bacterial moringa soap. Journal of Food Quality, vol.2023, issue.1, DOI: 10.1155/2023/5782063.
  2. Camilleri E, Blundell R. (2024). A comprehensive review of the phytochemicals, health benefits, pharmacological safety and medicinal prospects of Moringa oleifera, vol.10, issue.27807. Doi: 10.1016/j.heliyon. 2024.e27807.
  3. Salma Sultana (2020). formulation and evaluation of anti- bacterial moringa soap. Metabolism open, vol. 8. Issue.100061. https://doi.org/10.1016/j.metop.2020.100061
  4. Minakshi Jattan, N. Kumari, Raj Kumar, B. Rani, D. S. Phogat, S. Kumar, P. Kumar (2021). formulation and evaluation of anti- bacterial moringa soap. Journal of Horticultural Sciences. Vol.16, issue.1. https://doi.org/10.24154/jhs.v16i1.1073.
  5. Mallenakuppe, R., Homabalegowda, H., Gouri, M. D., Basavaraju, P. S., & Chandrashekharaiah, U. B. (2019). History, Taxonomy and Propagation of Moringa oleifera-A SSR Institute of International Journal of Life Sciences, 5(3), 2322–2327. DOI: 10.21276/SSR-IIJLS.2019.5.3.7.
  6. Authors: G.S. Dasat, G. Danjuma, E.S. Chundusu (2020). European Journal of Nutrition & Food Safety, vol.12, issue.10.  DOI: 10.9734/ejnfs/2020/v12i103030300.
  7. Charles Yameogo, Marcel Daba Bengaly, Aly Savadogo, Philippe A. Nikiema (2011), Pakistan Journal of Nutrition, vol.10, issue.3. DOI: 10.3923/pjn.2011.264.268.
  8. Bhattacharya, A., et al. (2018). Phytochemical and Pharmacological Characteristics of Moringa oleifera. Journal of Pharmacology & Pharmacotherapeutics, vol.10(4), issue.181–191. DOI: 10.4103/JPBS.JPBS_126_18.
  9. Chachapara, J., Chaudhari, K., Pandya, H., & Maitreya, B. (2022). Traditional and Advanced Use of Moringa oleifera as Food and Medicine. International Association of Biologicals and Computational Digest vol.2(1), issue. 130. DOI: 10.56588/iabcd. v2i1.130.
  10. Wijaya, D. F. H., Krisnawan, A. H., Yunita, O., Budi Pramana, K., Rani, K. C., & Jayani, N. I. E. (2023). Side Effect of Moringa oleifera Lamin Proceedings of the Conference on Natural Resources and Life Sciences (NRLS-BIO 2022,7–17). DOI: 10.2991/978-94-6463-322-1_2.
  11. Gupta, B. M., & Ahmed, K. K. (2020). Moringa oleifera: A Bibliometric Analysis of International Publications during 1935–2019.Pharmacognosy Reviews, vol.14(28), issue. 82–90. DOI: 10.5530/phrev.2020.14.12.
  12. Stohs, S. J., & Hartman, M. J. (2015). Review of the safety and efficacy of Moringa oleifera. Phytotherapy Research, 29(6), 796–804. DOI: 10.1002/ptr.5325.
  13. Limmatvapirat, C., et al. (2015). Comparison of eleven heavy metals in Moringa oleifera Lam. products. Indian Journal of Pharmaceutical Sciences, 77(4), 485–490. DOI: 10.4103/0250-474X.164782.
  14. Ayodele, O., et al. (2012). Toxicological evaluation of the aqueous leaf extract of Moringa oleifera Lam. (Moringaceae). Journal of Ethnopharmacology, 139(2), 330–336. DOI: 10.1016/j.jep.2011.11.008.
  15. Stohs, S. J., & Hartman, M. J. (2015). safety and efficacy of Moringa oleifera. Phytotherapy Research, 29(6), 796–804. DOI: 10.1002/ptr.5325.
  16. Coelho, J. S., et al. (2018). Moringa oleifera consumption during early life: Benefits and risks. Nutrition Reviews, 76(7), 487–500. DOI: 10.1093/nutrit/nuy001.
  17. Guilin Chen, Yongbing Xu, Jianlin Wu, Na Li, and Mingquan Guo (2021). Anti-Inflammatory Properties and Potential Bioactive Components from Moringa oleifera Leaves Revealed by Affinity Ultrafiltration LC–MS and Molecular Docking. Vol.1, issue.10. https://DOI.org/10.1021/acsfoodscitech.1c00275

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Anil Panchal
Corresponding author

Assistant Professor, Department of Pharmaceutics Delight college of pharmacy Koregaon Bhima, Pune. Maharashtra, India-412216.

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Nalawade Mahesh
Co-author

Research Scholar, Department of Pharmaceutics, Delight College of Pharmacy, Koregaon Bhima, Pune, Maharashtra. India-412216.

Photo
Vishal Madankar
Co-author

Assistant Professor, Department of Quality Assurance Techniques, Delight College of Pharmacy, Koregaon Bhima, Pune, Maharashtra, India-412216.

Nalawade Mahesh, Anil Panchal*, Vishal Madankar, Formulation and Evaluation of Anti- Bacterial Moringa Soap, Int. J. Sci. R. Tech., 2025, 2 (6), 233-241. https://doi.org/10.5281/zenodo.15597817

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