Abstract
Calanthe species is a Tujia ethnic herb, which has traditionally been used to treat gastric ulcers, chronic hepatitis, etc. We explored the chemical constitutes, gastroprotective effects, and the active fraction of C. species , as well as elucidating the underlying mechanisms. Firstly, four in vitro antioxidant tests were applied to determine the oxidation resistance of C. species methanol extract and its fractions. The gastroprotective effects were evaluated in ethanol-induced gastric ulcer rats, gastric histopathology was visualized by H&E staining, and the acidity of gastric juice was measured by titrating with NaOH solution. The contents of malondialdehyde, catalase, superoxide dismutase, gastroinstetinal, and the activity of H+K+-ATPase were estimated using commercial kits. For the first time, all of these compounds were extracted from C. Species . The findings showed that CsEF is a promising source of gastroprotective agents; the underlying mechanism of action was found to be the antioxidant activity of this herb, along with the prevention of gastrin secretion and inhibition of H+K+-ATPase.
Keywords
Medicinal plant, Therapeutic drug ,Secondary metabolites ,Calanthe species
Introduction
Calanthe species exist in the family Orchidaceous, the genus Calanthe contains over 220 species of orchids, which are usually referred to as Christmas orchids [1] These terrestrial evergreen or deciduous plants have big corrugated leaves, short oval pseudo bulbs, robust roots, and upright, occasionally arching flowering stems. Typically, the labellum has spreading lobes, and the sepals and petals are slender and comparable in size. Orchids in the genus Calanthe are terrestrial with small, crowded pseudobulbs with thick roots and a few corrugated or wrinkled leaves with the base tapering to a petiole -like stalk. Some species are evergreen while others are deciduous. The flowers are delicate but showy, white, pink, yellow or orange and crowded near the end of an erect, sometimes arching flowering stem. The sepals and petals are relatively narrow, similar in size and spread widely. The labellum has three or four spreading lobes and, in most species, there is a spur at the base. Unlike similar orchids, the labellum of Calanthe orchids is fused to the column. [2,3,4,5,6]
Reference
- Jones, David L.A complete guide to native orchids of Australia including the island territories. French forests, N.S.W.; New Holland. p353. ISBN1877069124. (2006).
- La Croix, Isobyl F. The new encyclopedia of orchids: 1500 species in cultivation. Timber press. ISBN9780881928761. (2008). p.78.
- Sasaki, Sanmi. Chado the way of Tea: A japanese tea Masters Almanac. Translated by Shaun McCabe; Iwasaki Satoko. Tuttle.ISBN978-0-8048-3716-3. (2005). pp.195-196
- Soon. Tech Eng. Orchids of Asia (3rd ed.) Times Editions – Marshall Cavendish ISBN9812610154. (2005). P.146.
- D.L. Jones; T. Hopley; S.M Duffy. ‘Calanthe’ Australian Tropical Rainforest Orchids. Centre for Australian National Biodiversity Research (CANBR), Australian Government Retrieved 27May 2021. (2010).
- Chen, Xinqui; Cribb, Phillip J.; Gale, Stephan W. ‘Calanthe’. Flora of China. Retrieved 8 September (2018).
- ab ‘Calanthe’ World Checklist of Selected Plant Families (WCSP). Royal Botanic Gardens, Kew.
- W3 Tropicos, Kew Monocot list. IPNI; The Orchids of Sikkim- Himalayas Part 1 King and Pantling 1898 drawing fide; AOS Bulletin volume 27/4 1958 drawing fide; Indian Orchids, Guide to indentification and Culture volume 2 Pardhan 1979drawing fide.
- King, G. & Pantling, R. (1898). The Orchids of the Sikkim-Himalayas. Ann. Roy. Bot. Garden. (Calcutta). Calanthe trulliformis King and Pantling, Page no 168.
- Bharali, P.; Das, A.K. & Liden, M. 2014. Notes on the alpine flora of Arunachal Pradesh, including several species new to India. In: Das, A.P. & Bera, S. (eds.), Plant Diversity in the 2007.
- T. Behl, A. Kotwani Anti-hyperglycemic effect of terminalia catappa fruit extract in streptozotocin-induced diabetic rats Int. J. Pharm. Pharm. Sci., 9 (2017), p. 212.
- R.N. Jangir, G.C. Jain Evaluation of antidiabetic activity of hydroalcoholic extract of Cassia fistula Linn. pod in streptozotocin-induced diabetic rats Pharmacogn. J., 9 (2017), pp. 599- 606
- T.O. Ijaola, A.A. Osunkiyesi, A.A. Taiwo, O.A. Oseni, Y.A. Lanreiyanda, J.O. Ajayi Antiiabetic effect of Ipomoea batatas in normal and alloxan-induced diabetic rats Iosr J. APP. Chem., 7 (2014), pp. 16-25
- G.M. Leung, K.S. Lam Diabetic complications and their implications on health care in Asia Hong Kong Med. J., 6 (2000), pp. 61-68.
- W. Li, M. Zhang, J. Gu, Z.J. Meng, L.C. Zhao, Y.N. Zheng, L. Chen, G.L. YangHypoglycem ic effect of protopanaxadiol-type ginsenosides compound K on Type 2 diabetes mice induced by high-fat diet streptozotocin via suppression of hepatic gluconeogenesis Fitoterapia, 83 (2012), pp. 192-198.
- S.A. Amiel, T. Dixon, R. Mann, K. Jameson Hypoglycaemia in Type 2 diabetes Diabet. Med., 25 (2008), pp. 245-254.
- R.J. Marles, N.R. Farnsworth Antidiabetic plants and their active constituents Phytomedicine, 2 (1995), pp. 137-189
- K. Miyoshi, M. MiiPhytohormone pre-treatment for the enhancement of seed germination and protocorm formation by the terrestrial orchid, Calanthe discolor (Orchidaceae), in asymbiotic culture Sci. Hortic., 63 (1995), pp. 263-267
- T. Nagashima On the Seed Germination and Embryogenesis in the Calanth furcata Bateman Calanthe cardioglossa Schltr, and Phaius minor Blume Engei Gakkai Zasshi, 52 (1983), pp. 65-77
- M. Yoshikawa, T. Murakami, A. Kishi, T. Sakurama, H. Matsuda, M. Nomura, H. Matsuda, M. Kubo p. p234-345.
- Novel indole S, O-bisdesmoside, calanthoside, the precursor glycoside of tryptanthrin, indirubin, and isatin, with increasing skin blood flow promoting effects, Cheminform, 46 (1998), pp. 886-888.
- Jin Hyun, J.Won Song, Nayoung Kwon, Young Kim, Park Soo Cost effectiveness associated with helicobacter pylori screening and eradication in patients taking nonsteroidal anti- Inflammatory drugs and/or Aspirin Gut Liver, 7 (2) (2013), pp. 182-189.
- Y. Jiang, H. Xu, T. Qin, W. Li, X. Wen, X. Yang, G. Feng, Y. Wei, H. Zhang An analysis on the volatile chemical composition in the leaves of Calanthe alpina by GC-MS pp. 56-58.
- Y. Jiang, H. Xu, W. Li, G. Dong, Y. Wei, M. Zhang, H. Wang, C. DengAntimicrobial test in vitro of Mayaqi extracts Shaanxi J. Tradit. Chin. Med., 36 (8) (2015), pp.1073-1074.
- Y. Peng, Y. Gao, X. Zhang, C. Zhang, X. Wang, H. Zhang, Z. Wang, Y. Liu, H. Zhang Antidiabetic and hepatoprotective activity of the roots of Calanthe fimbriata Franch Biomed. Pharm., 111 (2019), pp. 60-67.
- Hossain M.M. 2011. Therapeutic orchids: traditional uses and recent advances – an overview. Fitoterapia, 82(2): 102-140.
- Pant B. 2013. Medicinal orchids and their uses: Tissue culture a potential alternative for conservation. African Journal of Plant Science, 7(10): 448-467.
- Pérez Gutiérrez R.M. 2010. Orchids: A review of uses in traditional medicine, it phytochemistry, and pharmacology. Journal of Medicinal Plants Research, 4(8): 592-638.
- Verma R.S., Bhatia K.S. 1986. Chromatographic study of amino acids of the leaf protein concentrates of Ficus religiosa Linn and Mimusops elengi Linn. Indian Journal of Hospital Pharmacy, 23: 231–232.
- Taskeen A., Naeem I., Mubeen H., Mehmood T. 2009. Reverse phase high performance liquid chromatographic analysis of flavonoids in two Ficus species. New York Science Journal, 2(5): 32–35.
- Çimen Burak M.Y. 2008. Free radical metabolism in human erythrocytes. Clin. Chim. Acta, 390(1-2): 1–11.
- Singh, A. and Duggal, S. Ethnobotanical Leaflets, 2009, 13, pp. 351- 63.
- Clinical Laboratory Standard Institute, Performance Standard for antimicrobial susceptibility testing, M100-520, 2010, 30 (15).
- Acharya K. P. Orchid species richness along a Himalayan elevation gradient. Department of Biology, Faculty of Mathematics and Natural Sciences, University of Bergen, Bergen.pp 234.
Preeti Avasthi
Corresponding author
Department of pharmaceutical Chemistry, Maharaja Agrasen University, Kalu Jhanda Baddi (Himachal Pradesh) -173205
Nikita
Co-author
Department of pharmaceutical Chemistry, Maharaja Agrasen University, Kalu Jhanda Baddi (Himachal Pradesh) -173205
Nikita, Preeti Avasthi*, A Systemic Review on Calanthe Trulliformis, Int. J. Sci. R. Tech., 2026, 3 (2), 46-52. https://doi.org/10.5281/zenodo.18505703
10.5281/zenodo.18505703
